By default, this pipeline searches reference sequences using BLAST for user-provided fasta sequences from the local NR database downloaded from NCBI, and retrieves the sequence data of the three top hits. The unique sequences from the BLAST top hits, along with the input sequences are oriented according to the first sequence of the user provided dataset, then are aligned using MAFFT and the MSA is trimmed from both ends according to the first sequence. Further a phylogenetic tree is construction using RAxML-NG software. Please Note: Each module, i.e. homologous sequence search, sequence alignment and phylogeny building, of the pipeline can be used independently.